Analysis and Design of Stimulus Response Curves of E. coli

Analysis and Design of Stimulus Response Curves of E. coli

Blog Article

Metabolism and signalling are tightly coupled in bacteria.Combining several theoretical approaches, a core model is presented that describes transcriptional and allosteric control of glycolysis in Escherichia coli.Experimental Face Serums data based on microarrays, signalling components and extracellular metabolites are used to estimate kinetic parameters.

A newly designed strain was Cooler Bag used that adjusts the incoming glucose flux into the system and allows a kinetic analysis.Based on the results, prediction for intracelluar metabolite concentrations over a broad range of the growth rate could be performed and compared with data from literature.